Aryna Sabalenka defeated Jessica Pegula in the final, 6–3, 7–5, to win the women's singles tennis title at the 2024 Cincinnati Open.

[2] The two would play each other three weeks later, in the final of the US Open; Sabalenka would beat Pegula also in straight sets.

[3] Coco Gauff was the defending champion,[4] but lost in the second round[5] to Yulia Putintseva.

Under the 2024 WTA Rulebook, points from all combined ATP/WTA 1000 tournaments (which include Cincinnati) are required to be counted in a player's ranking.

† The player's 2023 points did not count towards her ranking on 12 August 2024 because the 2023 tournament was non-mandatory.