[1][2] Final 2024 roster[3][4] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

[5][6][7] For transfers in, dates listed are when Detroit City FC officially signed the players to the roster.

For transfers out, dates listed are when Detroit City FC officially removed the players from its roster, not when they signed with another club.

[18] The team earned their first home playoff game since joining USL Championship with their victory over Miami FC on October 19, and sealed third place in the Eastern Conference with a 4-1 win against San Antonio in the season finale.

[19][20] As a USL Championship club that finished below the top eight in the table in 2023, Detroit City FC entered the competition in the third round.