[2] Kawasaki Frontale were the defending champions, winning the 2023 final on penalties against Kashiwa Reysol.
On 19 September 2023, it was announced by JFA that Urawa Red Diamonds was banned from participating in this tournament following supporters clash after a 0–3 loss against Nagoya Grampus in the 4th round of the previous year's edition.
All teams playing in the 2024 J1 League (except Urawa Red Diamonds, who were disqualified) join in the second round.
As they were excluded from this year's cup, 2023 Japan Football League winners Honda FC filled the automatic entry slots for the second round.
Until 2011, it was an annual practice by JFA to give an automatic entry to Prime Minister's Cup champions.