The following parties and coalitions running in the European elections were represented in the Chamber of Deputies or in the European Parliament in time of the election: Alliance The following parties and coalitions have announced submitted their candidacy, were not represented in the Chamber of Deputies nor in the European Parliament at the time of the election, but have appeared at least one relevant opinion poll: The following parties and coalitions were not represented in the Chamber of Deputies or the European Parliament at the time of the election and have not appeared in any relevant opinion poll: The Civic Democratic Party (ODS), KDU-ČSL and TOP 09 have discussed whether they will run under the combined SPOLU banner or independently.

All incumbent MEPs (Mikuláš Peksa, Markéta Gregorová and Marcel Kolaja) announced their intention to run for the position of electoral leader.

[20] Former MP Mikuláš Ferjenčík also announced his candidacy, and received the endorsements of Minister of Foreign Affairs Jan Lipavský and former Mayor of Prague Zdeněk Hřib.

[23] Former Czech Prime Minister Jiří Paroubek has announced his intention to run in the election with his Nespokojení (Dissatisfied) movement, with the aim of connecting parties on the left of the political spectrum.

[29] From that date, no party or coalition can spend more than 50 million crowns on campaign promotion and must create a transparent bank account within five days.

The party also said it wants to curb illegal immigration, lessen the EU's impact on daily life, and will oppose the adoption of the Euro.

[32] The party announced its slogan as "Česko, pro tebe všecko" (English: "Czechia, everything for you"), accompanied by pictures of Babiš and Dostálová with Czech flags painted on their cheeks.

[33] SPOLU launched its campaign in Prague, in the café of Činoherní klub, where the Civic Forum was founded around 30 years earlier.

As its slogan, it chose "Bezpečná Evropa, silnější Česko" (English: "Safe Europe, stronger Czechia") and framed the election as a "clash between democratic parties and one-man projects".

He also said that wanted to replicate the "great Danish immigration model" and ease regulations concerning the manufacture of heavy weapons.

[36] Mayors and Independents launched their campaign before all other parties, with their leader Vít Rakušan starting a series of "Debates without censorship" in January, where he visited less-developed regions, mostly in the former Sudetenland, to answer questions from the local public.

[42] In April, Rakušan passed the debates to the leaders of STAN's candidate list, Jan Farský and Danuše Nerudová.

[45][46] As STAN's priorities, Nerudová mentioned fighting climate change, lowering the minimum voting age to 16, making the EU more accessible for young people, and preventing illegal migration.

[48] The Pirates launched their campaign on the last day of April, stating that they intend to focus on digitalization, improving quality of life, and fighting corruption and tax havens.

SPD said they want to attract voters by focusing on an economic issues, and by giving the lead spot to economist and former Svobodní MEP Petr Mach.