The 2024 Guadalajara 125 Open was a professional tennis tournament played on outdoor hard courts.

It was the 4th edition of the tournament and part of the 2024 WTA 125 tournaments (downgraded from WTA 250 status for the two previous editions).

It took place in Guadalajara, Mexico from 2 to 7 September 2024.

The following players received wildcards into the singles main draw: The following players received entry from the qualifying draw: ¹ Rankings are as of 26 August 2024.

The following pair received a wildcard into the doubles main draw: The following pair received entry as alternates: