The eight-team single-elimination tournament consisted of four rounds based on seeding from regular season conference play.
[3][4] The conference tournament title was the fourth for the Missouri State women's soccer program, and second for head coach Kirk Nelson.
The top eight of the eleven Missouri Valley Conference women's soccer programs qualified for the 2024 Tournament.
A tiebreaker was required to determine the second and third seed in the tournament as Drake and UIC finished with identical 6–2–2 regular season records.
A tiebreaker was required to determine the fifth and sixth seeds as Indiana State and Northern Iowa finished the regular season with identical 4–3–3 records.