Iga Świątek defeated defending champion Aryna Sabalenka in a rematch of the previous year's final,[1] 7–5, 4–6, 7–6(9–7) to win the women's singles tennis title at the 2024 Madrid Open.

[2] Sabalenka and Świątek became the first pair of players to contest consecutive finals at the tournament since the inception of the women's event in 2009.

Click on the seed number of a player to go to their draw section.

Seedings are based on WTA rankings as of 15 April 2024.

The following players would have been seeded, but withdrew before the tournament began.