The women's 400 metres at the 2024 World Athletics Indoor Championships took place in three rounds at the Commonwealth Arena in Glasgow, United Kingdom, on 1 and 2 March 2024.

This was the nineteenth time that the women's 400 metres was contested at the World Athletics Indoor Championships.

The two heats of the semi-finals were held in the evening of 1 March, where six athletes qualified for the final round.

The women's 400 metres was contested at every edition of the World Athletics Indoor Championships, eightteen times before the 2024.

[2] On 18 February 2024, Femke Bol of the Netherlands had broken the 400 metres short track world record in a time of 49.24 s at the Dutch Indoor Athletics Championships.

In the first heat, Lieke Klaver received a yellow card for improper conduct of disturbing the start and Sharlene Mawdsley was disqualified (DQ) for obstruction during the race.