Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.
[2][3][4][5] For transfers in, dates listed are when Detroit City FC officially signed the players to the roster.
For transfers out, dates listed are when Detroit City FC officially removed the players from its roster, not when they signed with another club.
[13][14] Detroit City was placed into group 4 for the group stage, alongside Hartford Athletic, Pittsburgh Riverhounds, Portland Hearts of Pine, Rhode Island FC and Westchester SC.
As one of the top four teams in the USL Championship Eastern Conference in the 2024 season, Detroit City will enter the tournament in the third round and be guaranteed to host the game at home.