[2] The competition will be played under the same format used in the most recent seasons, with a regular stage and a play-off tournament (known as Liguilla).
In the regular season, the 16 participating teams will play each other in a double round-robin tournament (once at home and once away) for a total of 30 matches, with the top team at the end of the 30 rounds winning the championship as well as promotion to the Chilean Primera División for the following season.
The team placing last at the end of the regular season will be relegated to the Segunda División Profesional.
[2] The tournament is played by 16 teams, 13 of them returning from the previous season, two relegated from the 2024 Campeonato Nacional (Cobreloa and Deportes Copiapó), and the 2024 Segunda División Profesional champions Deportes Concepción, who returned to the second tier after nine years following their disaffiliation from the ANFP in 2016.
[4] Deportes Concepción were ratified as champions by the ANFP on 4 February, greenliting the club's participation in the competition.