2025 USL League One season

Lexington SC left to join USL Championship,[6] Central Valley Fuego FC voluntarily left the league.

[7] The franchise agreement for the Northern Colorado Hailstorm FC was terminated when its owners filed for Chapter 11 bankruptcy.

[10] Union Omaha are the defending league champions and Player's Shield winners.

Each team plays the others twice, home and away, plus four additional matches.

The top eight teams at the end of the season will qualify for the playoffs.