The 224th Aviation was constituted on 1 October 1987 in the Maryland and Virginia Army National Guards as a parent regiment under the United States Army Regimental System, and organized from existing units to include the 1st Battalion and Companies D, E, and F, part of the 29th Infantry Division.

On 1 September 2003 the regiment was reorganized to eliminate Company F.[1] On 25 August 2005, the 2nd Battalion was ordered into Federal active service.

The two battalions of the regiment included elements from the Maryland, District of Columbia, Guam, Maine, Massachusetts, New Jersey, New York, Pennsylvania, Vermont, and Virginia Army National Guards as a result of the reorganization.

Additionally, a small contingent of six soldiers from the Oklahoma National Guard was attached to the 2nd Battalion from December 2005 until February 2007.

[1] The regiment was again reorganized on 1 September of that year; as a result, its two battalions included elements from the Maryland, District of Columbia, Maine, Massachusetts, New Jersey, New York, Pennsylvania, Tennessee, Vermont, and Virginia Army National Guards.