Constituted as the 26th Cavalry under the Combat Arms Regimental System in 1963, its 1st Squadron served with the 26th Infantry Division during the Cold War.

It was renumbered in 1988 as the 110th and reorganized under the United States Army Regimental System in 1988 before the 1st Squadron inactivated in 1996 as a result of reductions in the National Guard after the end of the Cold War.

It was organized on 1 March with the 1st Squadron, an element of the 26th Infantry Division.

Between 16 and 19 December 1967, the 1st Squadron was reorganized and Troop C was allotted to the Connecticut Army National Guard.

[4] The regiment was renumbered as the 110th Cavalry on 1 February 1988, with the 1st Squadron now solely a Massachusetts unit.