Initially the airbase operated solely Su-25s but in November 2007 it inherited some 20 Aero L-39 Albatros trainer aircraft from a disbanded unit.

[2] Between 4 April and 9 June 1980, the unit deployed three Yak-38s to Shindand Air Base, Herat Province, during the Soviet–Afghan War to test the aircraft's combat abilities.

On 2 July 2014, a Su-25, call sign Blue 06, crashed due to a technical fault while landing at Dnipropetrovsk International Airport.

[5][6] On the morning of 16 July 2014, Su-25 call sign Blue 03, was shot down over eastern Ukraine near Amvrosiivka town, and the pilot ejected successfully.

[10][11][12][13] Ukrainian Prime Minister, Arseniy Yatsenyuk, said later in an interview that one of the attack planes was probably shot down by an air-to-air missile.

The pilot, Captain Vladyslav Voloshyn, ejected and after 4 days was able to reach Ukrainian-controlled territory, was secured by a unit of the Ukrainian National Guard.

[22] During the war and until April 2023 some Su-25 stored or in reserve status were refited for combat operations and two more were acquired from the Republic of North Macedonia.

[58] On 10 April 2022, a Su-25 was destroyed in the ground inside a hangar at Dnipro Airbase by Russian rocket attacks, one Ukrainian technician was wounded as well.

[60][61] On 7 September 2022, Ukrainian media reported the death of 299th TAB pilot Vadim Blagovismy while flying a combat mission on a Su-25.

[68] On 7 February 2024 Ukrainian media reported the death of 299th Tactical Aviation Brigade pilot Vladyslav Rykov while flying a combat mission.