From 7 to 14 March 1776 the companies were organized from various counties from the eastern region of the colony of Maryland.

On 22 May 1777 the regiment was assigned to the 2nd Maryland Brigade in the main Continental Army.

The 2nd Maryland Brigade was reassigned to the Southern Department on 5 April 1780.

On 1 January 1781 the regiment was reassigned to the Maryland Brigade of the Southern Department.

the regiment was furloughed 1 January 1783 at Charleston, South Carolina and disbanded on 15 November 1783.