Companies were mustered in for three years service under the command of Colonel Horatio Gates Gibson.
2nd Brigade, 4th Division, XXIII Corps, Department of the Ohio, to February 1864.
2nd Brigade, 4th Division, District of East Tennessee, Department of the Cumberland, to March 1865.
Company A moved to Covington, Ky. 19 August 1863, then to Fort Jones, Muldraugh's Hill, Ky. 11 October, and duty there until 10 January 1864.
Company B moved to Covington Barracks 19 August, then to Bowling Green, Ky., 5 September, and duty there until 26 May 1864.
Company D moved to Muldraugh's Hill, Ky., and duty at Fort Saunders until 26 May 1864.
Company H moved to Munfordville, Ky. 11 October 1863, and garrison duty at Battery McConnell until 26 May 1864.
Company L moved to Frankfort, Ky., 11 October 1863, and duty at Fort Boone until December.
Ammon's Expedition to Bean's Station 7–29 December (Company A on duty at Knoxville until 9 January 1865.)
Company B on duty at Knoxville, Camp Rothrock, and Fort Saunders until August.
Company K at Clinch Gap until 21 December 1864, at Fort Lee Knoxville, and at Greenville until August.