It was converted into a signal aircraft warning regiment after the United States entered World War II.

[3] The 3rd Squadron and other 316th units in Vermont usually held inactive training period meetings at the Meade Building in Rutland.

In the fall or winter, Vermont and New Hampshire units conducted annual contact camps at Norwich University.

The 316th was converted into the 541st Signal Aircraft Warning Regiment on 30 January 1942, after the United States entered World War II.

The blue copied the color of one of the Tallmadge's Dragoons flags, and the Organized Reserve's Minuteman crest was located above the shield.