It is assigned to the 4th Operations Group and stationed at Seymour Johnson Air Force Base, North Carolina.

It was officially constituted by War Department letter on 12 August 1942, and was activated at Bushey Hall, England on 12 September 1942.

Currently, the squadron provides worldwide deployable aircraft and personnel capable of executing combat missions in support of worldwide Aerospace Expeditionary Force deployments to combat areas as part of the Global War on Terrorism.

Equipped with the Lockheed F-80 Shooting Star, the 334th moved to Andrews Air Force Base in April 1947.

Being on temporary duty, the 334th left their Thuds to be part of the developing 355th Tactical Fighter Wing, and in February 1966 returned to Seymour-Johnson.

Soon after the deployment to SEA, the operations officer, Maj Tokanel, lobbied for missions specifically flagged for the 334th TFS.

On 18 June 1991, the squadron became operational on the F-15E, and deployed to Saudi Arabia the next day to relieve elements of the 335th Fighter Squadron, providing combat air patrol and ground alert forces supporting withdrawal of troops from Operation Desert Storm

This article incorporates public domain material from the Air Force Historical Research Agency