By the end of the Korean War, the 335th led all squadrons with 218.5 kills (for around forty losses) and had become a part of the "MiG Killer" legend with 12 aces.

In May 1958, the squadron deployed to Eglin Air Force Base, Florida, tasked with operational testing of the new Republic F-105 Thunderchief for the next three years.

The squadron saw action in Southeast Asia in November 1965, flying the F-105 out of Takhli Royal Thai Air Force Base, Thailand.

In 1969, the 335th transitioned to the airframe its aircrew would fly for the next twenty-three years—the McDonnell F-4 Phantom II; later this year, they briefly deployed to South Korea.

In 1972, the squadron again saw action in Vietnam, from July to December, flying out of Ubon Royal Thai Air Force Base, Thailand.

On 27 December 1990, the 335th deployed twenty-four F-15Es along with support personnel and equipment to Al Kharj Air Base in central Saudi Arabia.

During the war, the squadron flew 1,097 combat missions over Iraq and occupied Kuwait, dropping over 4.8 million pounds of ordnance.

[2] After the war, the 335th continued to fly combat air patrol missions over Iraq and Kuwait until relieved by the 334th Fighter Squadron, departing on 24 June 1991.

In January 2002, the 335th deployed to Al Jaber Air Base, Kuwait in support of Southern Watch and Operation Enduring Freedom.

During Iraqi Freedom and Operation Southern Watch the squadron deployed 24 F-15Es, and flew 1,500 sorties, totaling 7,000 flying hours.

They dropped over one million pounds of precision and non-precision munitions on numerous targets such as key Iraqi leadership, command and control bunkers, artillery Republican Guard units and many others.