In the game, the player assumes control of a damned soul who must fight through Hell, Purgatory and Heaven as they rebel against a cruel God who banishes them.

Once a chamber is completed, players will unlock Relics and trigger God's wrath, which causes the entire map to be engulfed in fire.

The players are then returned to the Dark Forest in which they can change their loadout, discuss with non-playable characters to unlock new weapons, relics and poetic perks, which typically provide stat advantages.

For instance, Beatrice was reimagined from a guide in Purgatory to the "leader of the rebellion", and Virgil becomes a female scholar who is interested in "science, moral values, and the use of weapons".

[5] While preparing extensively is essential for players to succeed in raid missions in other MMO games, 33 Immortals was described by the team as a "pick up and raid" experience, as the game streamlines the mission process and allows players to jump into action "as quick and seamless as possible".