The regiment was formed on the basis of the 10th Separate Brigade of Special Purpose of the Main Reconnaissance Directorate (GRU) of the Soviet Armed Forces.

July 29, 2014 became one of the most tragic days for the regiment, when the largest number of special forces died at the same time.

Led by captains Taras Karpa and Kyril Andriyenko, reconnaissance groups, a total of 19 fighters while carrying out a pilot evacuation operation for the downed Ukrainian Su-25 aircraft got ambushed near the village of Latysheve in Donetsk region.

One special forces officer was killed, eight were wounded, two Mi-24s had to be called to help, which dealt a devastating blow to the militants.

[4] On December 29, 2019, four Ukrainian special forces were released from Russian captivity as a result of the prisoner exchange.

During the same exchange, Ukraine handed over farmer Volodymyr Butrymenko, who was guilty of organizing an ambush against Ukrainian special forces on July 29, 2014.

[5] On 6 December 2021, to the 30th anniversary of the creation of the Armed Forces of Ukraine, 13 light armored HMMWV vehicles provided by the United States as part of logistical assistance were transferred to the 3rd Separate Special Purpose Regiment.

[9] The 3rd separate special purpose regiment took part in the Battle of Avdiivka from the beginning to the end of the defensive operation.

Within a week in April 2024, in the Donetsk direction the operators of FPV-drone unit of the tactical group "Medoid" destroyed a Russian T-72 tank, anti-aircraft missile cannon complex 2C6 "Tunguska", an armored vehicle along with 11 enemy troops.

On June 12, 2024, for the first time during the war the 3rd Special Operations Forces Regiment destroyed the newest Russian R-416GM digital radio relay communication station, a truck-based system designed to improve the efficiency of radio relay units in the field.