It mustered in for three years service April 1862 under the command of Colonel Lewis T. Campbell.
The regiment was attached to District of Nashville and Franklin, Unattached, Army of the Ohio, to September 1862.
29th Brigade, 8th Division, Army of the Ohio, to November 1862.
2nd Brigade, 2nd Division, Center, XIV Corps, Army of the Cumberland, to January 1863.
The 69th Ohio Infantry mustered out of service at Louisville, Kentucky on July 17, 1865.
Moved to Camp Chase, Ohio, February 19, 1862, and duty there until April 1862.
Moved to Nashville, Tenn., April 19–22, thence to Franklin, Tenn., May 1, and duty there until June 8.
Expedition to Gallatin and action with Morgan August 13.
Battle of Stones River December 30–31, 1862 and January 1–3, 1863.
Passage of the Cumberland Mountains and Tennessee River, and Chickamauga Campaign August 16-September 22.
Veterans absent on furlough March 16-May 11, rejoin at Buzzard's Roost, Ga. Atlanta Campaign May to September.
Operations on line of Pumpkin Vine Creek and battles about Dallas, New Hope Church and Allatoona Hills May 25-June 5.
Moved to Louisville, Ky., June, and duty there until July The regiment lost a total of 187 men during service; 5 officers and 84 enlisted men killed or mortally wounded, 98 enlisted men died of disease.