It was formed in August 1862 as a response to the outbreak of the American Civil War, being mustered in for three years of service and to deter the Confederate Army and achieve the goals of the Union.

Organised on August 19, 1862, under Colonel William L. McMillen, it proceeded to Kentucky and engaged in the Battle of Richmond where most of the regiment was captured.

This was an utter disaster for the regiment, the men were eventually exchanged in November, and until May 1863 were kept at work in drill and reorganisation.

The Regiment was finally mustered out on August 19, 1865, after sustaining a loss of over 500 men during its term of service.

[2] According to documentation and reports, the following structure of the regiment was as follows: According to the Civil War Soldiers and Sailors database, it is able to list approximately c1700+ men on its roster for the 95th Ohio Infantry Regiment [3]