Heavy goods vehicles and those over 14 feet 6 inches (4.42 m) are prohibited from parts of the route (due to a low underpass at Brampton Road).

From the Swavesey interchange to Bar Hill the former A14 Eastbound carriageway is utilised, including a new grade separated junction for Lolworth.

From Bar Hill to the Girton interchange (the old northern terminus of the A1307), the A1307 follows local access roads (opened in 2019) running parallel to the A14.

The road then passes the Babraham business park and carries on to Abington, Fourwentways interchange with the A11 and services provided by Euro Garages and a hotel by Travelodge.

Many fatal accidents have occurred along the route, particularly between Little Abington and Horseheath (close to the Cambridgeshire/Suffolk border), leading to frequent pressure from regular users to have safety improvements added.