The A28 traverses the Dutch provinces of Utrecht, Gelderland, Overijssel, Drenthe and Groningen.

The road connects the cities of Utrecht, Amersfoort, Zwolle, Assen, and Groningen.

Major motorway junctions can be found at the interchanges Rijnsweerd near Utrecht (A27), Hoevelaken near Amersfoort (A1), Hattemerbroek near Zwolle (motorway A50 / highway N50), Lankhorst near Meppel (A32) and Hoogeveen near Hoogeveen (A37).

The northern terminus is at the traffic light controlled intersection of Julianaplein near Groningen (motorway A7 / highway N7).

Between the interchange Hoevelaken near Amersfoort and the terminus at Julianaplein in Groningen, the A28 is also a European route: the E232.