She is homeported at Puerto Belgrano Naval Base and is part of the Navy's 2nd Corvette Division with her five sister ships.

On 25 September 1990, Spiro and the destroyer Almirante Brown participated as part of a multinational task force in the United Nations-mandated blockade of Iraq following its invasion of Kuwait.

Spiro also participated in several naval exercises and conducted fishery patrol duties in the Argentine exclusive economic zone, capturing three illegal fishing ships between 1991 and 1994.

[2] This meant that her sister ARA Espora had to make an unscheduled deployment to replace Spiro on the Atlasur IX exercise off West Africa.

[2] In 2021, Spiro was reported active and, in September, participated in a sea exercise also involving her sister ships Espora, Robinson and Gómez Roca, along with the destroyer Sarandí.