[4] Graduating from the youth setup of Bunyodkor, Khusanov started his career at Belarus with Energetik-BGU in 2022, where he played for one and a half seasons before his transfer to French side Lens in 2023.

[9] In August, Khusanov was included in the list of the most promising players in the world according to the International Center for Sports Research.

[18] He made his debut for the club on 16 September in a 1–0 home loss against Metz,[19] becoming the first Uzbek player to play in Ligue 1.

[21] His final match with Lens took place on December 22, 2024, in the Coupe de France against Paris Saint-Germain, marking his debut as the first Uzbek player to participate in the competition.

[22] On 20 January 2025, Khusanov became the first Uzbek player to sign for a Premier League club, joining Manchester City on a four-and-a-half-year deal, on an initial reported fee of €40 million.

[26] On 8 February 2025, Khusanov inadvertently scored his first goal for Manchester City on his FA Cup debut in a 2–1 away victory over EFL League One side Leyton Orient in the fourth round when the ball deflected off his head from a shot by teammate Rico Lewis.

On 11 March 2023, Khusanov helped the team defeat Australia in a penalty shoot-out in the quarter-finals, thus securing a spot in the 2023 FIFA U-20 World Cup.

[32] Together with the team, Khusanov reached the CAFA Nations Cup final, securing first place in the group stage.

He ultimately became a silver medalist in the tournament, as the team lost to Iran in the final on 20 June, where Khusanov spent the entire match on the bench.

Together with the team, he reached the quarter-finals, where Uzbekistan were eliminated by Qatar on 3 February in a penalty shoot-out, thus concluding their tournament run.

[35] Khusanov is the son of Hikmat Hashimov (also transliterated as Khikmat Khashimov), who had a brief stint as a professional football player with PFK Metallurg Bekabad.