Acequia Madre de Valero is an 18th-century agricultural irrigation canal built by the Spanish and located in the Bexar County city of San Antonio in the U.S. state of Texas.

Acequia Madre de Valero ran from the area currently known as Brackenridge Park southward to what is now Hemisfair and South Alamo Street.

[4] Acequia Madre de Valero was the initial phase of what became a 45-mile network put in place by the Franciscan priests to provide water for the missions and their agricultural endeavors.

Part of this system is adjacent to the Johann and Anna Heidgen House at 121 Star Street, and was a contributing factor in placing the house on the National Register of Historic Places listings in Bexar County, Texas in 2004.

[6] Media related to Acequia Madre de Valero at Wikimedia Commons See also: List of museums in Central Texas