He was married twice, first to Hedevig Ulrikke Luxdorph and second time to Ida Margrethe von Reventlow.

His mother converted Årsmarke into a countship for her eldest son when, in 1714, Adam Christoffer von Knuth was elevated to the status of a count under Frederick IV which resulted in the change of the estate to Knuthenborg.

They had one child, the daughter Frederikke Louise, Countess Knuth (1720–1793), who would later marry Vilhelm lensbaron Güldencrone (1701–1747).

They were married on 16 December 1721 and had the following children: Adam Christopher Knuth died on 23 January 1746 and was buried in Hunseby Church.

She also converted Rosengaard, Sandbygaard and Sørupgaard into a barony under the name Vonradsborg for their son Conrad Knuth.