[2] Studies of Aedes circumluteolus in the floodplains of northern KwaZulu-Natal in South Africa found that the species' population density reached its highest levels after the plains were inundated by the Pongola and Maputo Rivers, and that changes in the population density were influenced much more by river flooding than local rainfall.

During dry months, males of the species mostly disappear while females persist at very low levels; blood-feeding and ovarian development occur throughout the year, with antelope as the preferred host in this area.

[3] In settled areas, the females have been noted to feed on humans, oxen, goats and dogs.

Immature stages of Aedes circumluteolus may be found in shallow temporary pools, and the species overwinters mainly in the form of drought-resistant eggs.

The eggs' micropylar apparatus is unusual in that the disk may be covered with a dense mat of fine filamentous strands connected to small, sharp, irregular papillae.