[2] The chapel is located on the southern half of Oudezijds Voorburgwal canal, the so-called stille zijde ("quiet side").

After the Alteratie in 1578, when the Catholic city government was deposed in favor of a Protestant one, all monasteries and convents were closed and most were demolished.

The ground floor of the former chapel was used as an arsenal of the Admiralty of Amsterdam until the construction of 's Lands Zeemagazijn (now the National Maritime Museum) in 1656.

In the chapel attic, a stadsboekerij ("city bookery") was opened in 1632, the predecessor of the current Amsterdam University Library.

When the Athenaeum Illustre outgrew the chapel, it moved in 1864 to the Handboogdoelen on the Singel canal, a former shooting range of the Amsterdam schutterij (civic guard).