Ahafo Ano South District is a former district that was located in Ashanti Region, Ghana.

Originally created as an ordinary district assembly in 1988, which was created from the former Ahafo Ano District Council.

However, on 15 March 2018, it was split off into two new districts: Ahafo Ano South West District (capital: Mankranso) and Ahafo Ano South East District (capital: Adugyama).

The district assembly was located in the western part of Ashanti Region and had Mankranso as its capital town.

This Ashanti Region location article is a stub.