Born in Tallinn, Ain Lutsepp began his career as a child actor at age thirteen as the character Tõnisson in the 1969 Arvo Kruusement directed Estonian language film Kevade (English: Spring); a film adaptation of author Oskar Luts' popular 1913 novel of the same name.

[3] Graduating classmates included actors Roman Baskin, Guido Kangur, Arvo Kukumägi, Paul Poom, Anne Veesaar, and Ülle Kaljuste.

Among his more memorable international roles in theater were in works by: William Shakespeare, Lev Tolstoy, Charles Dickens, Jean Genet, Tom Stoppard, Samuel Beckett, Anton Chekhov, Henrik Ibsen, Arthur Miller, and Eugene O'Neill, among others; in addition to works by Estonian playwrights and authors A. H. Tammsaare, Madis Kõiv, Jaan Kross, Bernard Kangro, Paul-Eerik Rummo, Juhan Smuul, Hugo Raudsepp and others.

[5] Other film roles include that of Madis in the 1983 biopic Lurich, about the Estonian strongman Georg Lurich; Jaan Lõoke, in the 1983 film Nipernaadi, an adaptation of August Gailit's 1928 novel Toomas Nipernaadi; Nymann, in the 2004 Hardi Volmer directed historical thriller Tulivesi (English: The Firewater); Julius Saarepuu, in the 2008 historical drama Detsembrikuumus (English: December Heat); as Ants, in the 2012 Ain Mäeots directed drama Deemonid (English: Demons), and as Volli in the Sulev Keedus-directed drama Mehetapja/Süütu/Vari in 2017.

Additionally, he voiced the character of Eduard in the 2006 Estonian animated film Lotte from Gadgetville, as well as having performed in several radio plays.