It was also known for a time as Owensboro Aviation.

It began operations in 1974, and joined the Allegheny Commuter system for Allegheny Airlines, and later USAir Express.

The airline was mentioned in the film The Life Aquatic with Steve Zissou; one of the main characters, Ned Plimpton, is a pilot for Air Kentucky, however, in the film Ned mentioned that the airline was based out of Louisville, Kentucky.

Those airports marked with an asterisk (*) are no longer served by commercial airline service.

This article relating to a United States airline is a stub.