Aisey-sur-Seine (French pronunciation: [ɛzɛ syʁ sɛn], literally Aisey on Seine) is a commune in the Côte-d'Or department in the Bourgogne-Franche-Comté region of eastern France.

The commune can also be accessed from the north on Highway D971 (the Route de Dijon) from Nod-sur-Seine.

The commune has large areas of forest - especially in the east and the south with some farmland on the western side.

The Brevon Stream which forms the south-eastern boundary of the commune joins the Seine near the intersection of Highway D29 and D101 (a side road from the D971).

List of Successive Mayors of Aisey-sur-Seine[5] The inhabitants of the commune are known as Aiseyens or Aiseyennes in French.