He was ordained to the priesthood on November 1, 1931, by Bishop Reginald Heber Weller of the Episcopal Diocese of Fond du Lac.
He served at two small churches in Wisconsin before his term as canon pastor of St. Paul's Cathedral, Fond du Lac, from 1935 to 1938.
He was also rector twice of the Church of the Ascension and St. Agnes in Washington, D.C., serving from 1938 to 1941 and 1946 to 1950, with significant interruptions for military service under General George S. Patton in the European Theatre.
In 1976 duBois became president of Episcopalians United, which was opposed to revision of the 1928 Book of Common Prayer and the ordination of women; the American Church Union declared this organization to be defunct in September 1976.
Following the September 1976 General Convention resolutions in favor of the 1979 Book of Common Prayer and the ordination of women, duBois renounced his orders in the Episcopal Church.