He previously played for Příbram, Viktoria Plzeň, Brighton & Hove Albion, Brescia, Venezia and Palermo, and has represented his country at all underage levels.

However, his status in the team was downgraded in the 2016–17 season as Radim Řezník took his place as the first-choice right back.

[2] He later signed a three-year contract with English Premier League club Brighton & Hove Albion on 4 August 2017.

[5] On 10 February 2022, Matějů signed with Venezia until the end of the 2021–22 season, with an option to extend for two more years.

[6] On 26 August 2022, having been released by Venezia, Matějů signed for Serie B club Palermo as a free transfer, reuniting with his former Brescia coach Eugenio Corini.