[1] Allori supplemented his training with a study trip to Rome, between 1554 and 1560, and with anatomical research which included the dissection of human corpses, provided by the Hospital of Santa Maria Nuova.

He contributed four painted panels: a Banquet of Cleopatra, a landscape with figures diving for pearls, and portraits of Cosimo I de' Medici and Eleanor of Toledo, the parents of Francesco I.

[4] Between 1578 and 1582 he worked in the Medici Villa di Poggio a Caiano, expanding a fresco of Tribute to Caesar which Andrea del Sarto had painted in the 1520s.

The art historian Simona Lecchini Giovannoni is more positive, remarking that Allori gives life to these "grandiose, introverted figures" by surrounding them with realistic depictions of plants and flowers, household furniture, and textiles; the paintings "approach the spectator, not with dialogue and sentiment, but through the tangible evidence of objects and details".

Cristofano dell'Altissimo, Cesare Dandini, Aurelio Lomi, John Mosnier, Alessandro Pieroni, Giovanni Battista Vanni, and Monanni also were his pupils.