His co-ownership deal was terminated in the favour of Bologna in June 2002, for a peppercorn fee, making the Turin side register a loss of €1.5 million (the residual unamortised transfer fee of €4.64 in three years), but with a financial income of €2.32 million as Juve did not have to pay the remaining 50% registration rights.

[1] Following Bologna's relegation in 2005, Gamberini moved to ACF Fiorentina for €3.2 million,[2][3] carving a starting XI niche in his second year as the Viola eventually qualified twice for the UEFA Champions League.

[10] He scored his first and only league goal for them on 11 September 2016, helping the hosts earn one point following the 1–1 home draw to Lazio.

[14] Gamberini was not included in the original squad for UEFA Euro 2008, but was called up after captain Fabio Cannavaro tore ankle ligaments after a collision with teammate Giorgio Chiellini in training, on 2 June.

[15] Gamberini was selected by Marcello Lippi for the 2009 FIFA Confederations Cup tournament, but once again failed to make an appearance as the nation was eliminated in the first round.