She played the lead role of Olivia Murray in the series Above the Law, and appeared as Kelli Edwards in Home and Away.

As a presenter, she hosted the series Sex/Life, co-hosted Australia’s Funniest People, and was a regular on Home Life Style, Beauty and the Beast,[1][2] and Good News Week.

In the science fiction series Farscape she appeared as the character Gilina Renaez in the episodes "PK Tech Girl", "Nerve", "The Hidden Memory", and "John Quixote".

On stage, Cook played Columbia in The New Rocky Horror Show 1992 Australian tour, and has appeared in The Vagina Monologues.

Alyssa-Jane Cook lives in Sydney with her husband Gary Davis and their three children, including Georgia-May, who was the oldest cast member of the 2012 production of Young Talent Time on Channel Ten and stars in Dive Club.