Amalie was born in Mannheim, the daughter of Count Palatine Frederick Michael of Palatinate-Zweibrücken-Birkenfeld-Bischweiler and his wife, Countess Palatine Maria Franziska of Pfalz-Sulzbach.

On 29 January 1769 she married the Saxon Elector Frederick Augustus III.

The following year, Napoleon I made them Duke and Duchess of Warsaw, a newly created principality in Poland.

Only one daughter, Princess Maria Augusta, attained adulthood, but remained unmarried.

[1] Amalie died on 15 November 1828 at the age of 76, and was buried in the Hofkirche in Dresden.