At the time he was also responsible for the classes of mathematics and sciences at the Colegio Santa Tereza, a Roman Catholic school run by nuns in Ituiutaba.

He collected most of his plant material in the cerrado vegetation of the States of Minas Gerais, Goiás, Maranhão and Pará.

He travelled all over the cerrado region and wrote diaries of his trips in which he describes the plants, the environment, the villages, the customs of the people, the food, the transport, the rivers and so on.

He learned from and kept an exchange of correspondence with Brazilian botanists, such as Joaquim Franco de Toledo, Oswaldo Handro, Frederico Carlos Hoehne, Graziela Maciel Barroso, Carlos de Toledo Rizzini, Alexandre Curt Brade, Guido Frederico João Pabst, Gil Martins Felippe, and Lúcia Rossi e João Aguiar Nogueira Batista.

Legrand, from Uruguay, Lorenzo R. Parodi and Arturo E. Burkart, from Argentina, Harold N. Moldenke, Richard Sumner Cowan, Robert E. Woodson Jr., Conrad V. Morton, Jason R. Swallen, and Lyman B. Smith, from the United States, Noel Y. Sandwith, from England, Joseph V. Monachino, an Italian working in the United States, and Erik Asplund, from Sweden.

Acanthaceae Amaryllidaceae Asclepiadaceae Bignoniaceae Bromeliaceae Compositae Connaraceae Gramineae Labiatae Liliaceae Melastomataceae Velloziaceae Acanthaceae Aspidiaceae Bromeliaceae Compositae Convolvulaceae Dryopteridaceae Gramineae Lauraceae Leguminosae–Caesalpinioideae Leguminosae–Mimosoideae Leguminosae–Papilionoideae Malpighiaceae Malvaceae Melastomataceae Myrtaceae Ochnaceae Onagraceae Opiliaceae Orchidaceae Piperaceae Polypodiaceae Rubiaceae Rutaceae Velloziaceae Verbenaceae Viscaceae Media related to Amaro Macedo at Wikimedia Commons