Founded in 1927 as part of the football team Ambrosiana-Inter of Milan, the club won the first ever Italian championship in 1929 and ever since have won 18 domestic championships that made them Italy's most titled rugby union team.

Between 1993 and 1997 Amatori Milano was part of the A.C. Milan sport club under the ownership of Silvio Berlusconi and won 4 domestic championships.

The club were revived in 2002 and coached by Marcello Cuttitta, a former Italian international rugby union player that played for Amatori in the 1990s, managing to reach the second tier of Italian rugby union championship before being disbanded in 2011.

Amatori Rugby Milano would dominate the coming years, winning back-to-back titles in 1931, 1932 and 1933.

In 2010 Amatori Milano is promoted to Serie A, the second tier of Italian rugby union championship, where they competed in two seasons before being disbanded in 2011.