Covering an area of 4,425 km² (1,709 sq mi), it is protected from human settlements, hunting, mining and deforestation, though problems with all these still exist within the park.

Amboró National Park is in the western part of Santa Cruz Department, at the "Elbow of the Andes", where the eastern cordillera bends slightly westward from its northern course.

[1] The peculiar features of the geography of the Amboró park area determine the biological makeup, with a great variety of flora and fauna.

Today it has returned to gravel and dirt, limiting traffic and commercial exchange on south side of Amboró National Park.

Off of these two roads are a number of secondary gravel ones, allowing access to the more developed parts of the northern and southern Integrated Management Natural Areas or IMNAs.

With funding from the Regional Alternative Development Program (Programa de Desarrollo Alternativo Regional - PDAR), a consensus was finally reached with the establishment of two different management categories, indicated on the ground by a so-called "Red Line", a narrow trail that marks the boundary between the National Park and the Integrated Management Natural Zone (IMNA), effectively a Multiple Use Zone.

In 1994, FAN was selected by the newly created Ministry of Sustainable Development and Environment to supervise the drafting of the area's management plan.

Much of the biodiversity of Amboró is partitioned according to the park's highly dissected topography and the widely varying climatic regimes that accompany the sharp transitions in elevation.

The ecotones between these vegetation types are often sharply delineated according to aspect and elevation, yielding a highly heterogenous landscape that can make traveling in the park a dramatic and surprising experience.

Another factor contributing to the park's high plant species richness is its location at the confluence of several diverse and unique floristic regions: the tropical Amazon lowlands and pampas to the north and southeast, the subantarctic high Andes and altiplano to the west and southwest, the subtropical Tucumano-Boliviano forests to the south (these forests do not actually reach Amboró but many of their constituent species occur within the park), and the semiarid inter-Andean valleys and wet-tropical yungas forests that characterize the eastern slopes of the central Bolivian Andes.

Of the documented species, some of the most ecologically and economically interesting are bigleaf mahogany, mountain pine, black walnut (no relation to the eastern North American tree of the same name), limachu, q'illu q'illu (Berberis bumaelifolia, also spelled khellu khellu), cebillo, bibosi, ambaiba, pacay, and clavo rojo.