Ambroise-Dydime Lépine (18 March 1840 – 8 June 1923) was a Métis politician, farmer, and military leader under the command of Louis Riel during the Red River Rebellion of 1869–1870.

On 30 October, he learned of plans to transfer Rupert's Land to Canada from the Hudson's Bay Company and that the resident Métis, led by Louis Riel, were seeking to negotiate terms for their annexation.

After meeting Riel, Lépine was instructed to go with 14 men to Pembina, Dakota Territory, and prevent Lieutenant Governor–Designate William McDougall from crossing the Canada–United States border.

[3] On 8 January 1870, the Provisional Government of Manitoba under Riel named Lépine adjutant general of St. Boniface, whose populace elected him to a 40-man convention in the city and to head its military council.

[3] When Colonel Garnet Wolseley arrived in the Red River Colony with his forces on 24 August 1870, Riel and Lépine fled to a Catholic mission in the U.S. Dakota Territory.