[3] This species is threatened by habitat loss due to agriculture, gold mining, oil extraction, road construction, and logging.

[2] Ameerega shihuemoy is a terrestrial frog which primarily inhabits fast-flowing rocky streams, and low disturbance forested areas in the Andean foothills of the Manú District.

In areas of low disturbance they can also be found near wetlands, and during the rainy season near small temporary pools of standing water.

Females deposit clutches of 20–25 eggs inside of rock shelters near permanent and seasonal streams.

One such individual was observed at the Manú Learning Centre carrying a group of 10 tadpoles on its back along a stream.

Reproduction is believed to take place during both the dry and rainy seasons, as mating calls can be heard throughout the year.

Both species tadpoles are found in streams with shallow, slow moving, clear water with a sediment of sand and dead leaves.

Calling typically occurs most prominently during the early morning between 5:00-9:00 AM and late afternoon and dusk between 4:00-6:00 PM.

[2] The Amarakaeri poison frog is a medium-sized dart frog with a black unmarked dorsum, and an orange dorsolateral stripe running from the tip of the snout to the groin where it turns into a bright orange blotch that extends slightly to the anterior surface of the thigh.

Under the eye the frog has a whitish-bronze labial stripe which extends under the tympanum to the base of the upper arm.