Neischnocolus species have modified Type I urticating hairs on the abdomen, similar to those of Proshapalopus and Citharacanthus livingstoni.

[2] The genus Ami was erected in 2008 by Fernando Pérez-Miles;[1] the name is based on a word in the Tupí language, meaning "spider that does not spin a web".

Ami seemed to be more closely related to the genus Proshapalopus than to other genera of the large subfamily Theraphosinae.

[2] The six new species were A. caxiuana, named after the type locality, which means "place of many snakes" in Tupí; A. yupanquii, named after the Inca leader Tupac Yupanqui, who unified the agricultural populations of Ecuador; A. bladesi named in honor of Panamanian singer and composer Ruben Blades; A. pijaos, honoring the Pijaos, an ancient culture that populated the region of the type locality; A. amazonica, referring to the Colombian Amazonic region; and A. weinmanni, named after Dirk Weinmann, the collector of the type specimens.

[2] In 2019, Pérez-Miles and co-workers discovered that Ami bladesi was identical to the earlier described Neischnocolus panamanus Petrunkevitch, 1925, whose holotype had been rediscovered.