His orquesta típica was among the most popular with social dancers during the golden age of tango (1940–1955), but he changed to a concert sound by the late 1950s.

Troilo's orchestra is best known for its instrumentals, though he also recorded with many well-known vocalists such as Roberto Goyeneche, Edmundo Rivero and Francisco Fiorentino [es].

In December 1930, Troilo was hired to be part of the famous Vardaro-Pugliese sextet of violinist Elvino Vardaro, pianist Osvaldo Pugliese, violinist Alfredo Gobbi, bandoneon player Miguel Jurado (later replaced by Ciriaco Ortiz), and double bass player Luis Addesso.

In 1968, having distanced himself from Grela, he formed his own ensemble, the Aníbal Troilo Quartet, which additionally consisted of Ubaldo de Lío on electric guitar, Osvaldo Berlingieri on piano, and Rafael del Bagno on double bass.

The "Supreme Bandoneón of Buenos Aires" died on 18 May 1975, at the Italian Hospital of Buenos Aires, from a stroke and subsequent cardiac arrest; he is buried in the "Rincón de los Notables" (Famous corner) of the La Chacarita cemetery, next to Agustín Magaldi and Roberto Goyeneche.