Her father owned a furniture store and a theater that would provide Mariscal much of her early exposure to acting.

[1][2] Ana Mariscal began her career after accompanying her actor brother Luis Arroyo to an audition for El Ultimo Husar.

A few notable films from this time include The Queen's Flower Girl, Raza, A Shadow at the Window, and The Princess of the Ursines.

[6] In her later career, she shifted among film, television, and theater pursuits, still using her production company to fund her projects.

She began teaching classes at the IIEC (Institute of Investigations and Cinematographic Experiences) while still balancing acting and directing jobs.