Regarded as the "Father of Philippine Science and Laboratory", del Rosario invented the formula for producing a pure kind of alcohol from tuba in a nipa palm.

This formula won for del Rosario the first prize during the World Fair in Paris, France in 1889.

His position lead him to establish the beginnings of Science and technology in the Philippines by increasing the standards for quality control on essential items and investigation of natural resources.

[2] Anacleto del Rosario first studied in a private institution, and his rather stern relative was his guardian who supervised his schooling.

In return for the tutelage of his guardian, Anacleto had to wash the bottles in his Aunt's drugstore.

Accounts from his son Jose relates that he escapes through the window of the bathroom of their own home just to go out and borrow books from classmates.

During his years in Ateneo, he made and sold electric bells, household fixtures, and toys to earn money.

He gained his Bachelor in Arts degree on April 1, 1876, with the highest honors (Nota Maxima).

Using the money earned from surveying, he decided to buy a microscope and books relating to microbiology.

On June 17, 1882, he was appointed as a pharmacist member (Vocal farmaceutico) of the Commission of Health.

[2] In 1886, Anacleto del Rosario took the examinations at the Manila Mint (Casa de Moneda).

[2] He married into Valeriana Valdezco even if her parents initially disapproved of him due to his financial condition.

He had three children altogether He was described in his youth as a gentle and sweet person in deposition.